Synthesis of a Designer Eukaryotic Chromosome

Total Synthesis of a Functional Designer Eukaryotic Chromosome
Science. 27 March 2014

Rapid advances in DNA synthesis techniques have made it possible to engineer viruses, biochemical pathways and assemble bacterial genomes. Here, we report the synthesis of a functional 272,871–base pair designer eukaryotic chromosome, synIII, which is based on the 316,617–base pair native Saccharomyces cerevisiae chromosome III.
Changes to synIII include TAG/TAA stop-codon replacements, deletion of subtelomeric regions, introns, transfer RNAs, transposons, and silent mating loci as well as insertion of loxPsym sites to enable genome scrambling.
SynIII is functional in S. cerevisiae. Scrambling of the chromosome in a heterozygous diploid reveals a large increase in a-mater derivatives resulting from loss of the MATα allele on synIII.
The complete design and synthesis of synIII establishes S. cerevisiae as the basis for designer eukaryotic genome biology.

related articles:

Scientists Move Closer to Inventing Artificial Life
Synthetic biology advances with a man-made chromosome inserted into live brewer’s yeast.
National Geographic. March 27, 2014

Student Assembly Drives Yeast Project
Science 28 March 2014: 343(6178):1429 (PAID)

Building the Ultimate Yeast Genome
Elizabeth Pennisi
Science 28 March 2014: 1426-1429. (PAID)

The Science of Genius

The Science of Genius
Outstanding creativity in all domains may stem from shared attributes and a common process of discovery
Scientific American Mind. Special Edition: Creativity
Winter 2014. pp. 20-27
By Dean Keith Simonton


Sudden Genius?
Does Genius Follow The Ten-Year Rule?
Psychology Today. February 1, 2011

The Art of Medicine: Perspiration, inspiration, and the 10-year rule Vol 376 October 30, 2010
the 10-year rule was first identified by psychologist John Hayes in 1989

Science and Fiction – A Springer Series

Science and Fiction – A Springer Series

Readers can look forward to a broad range of topics, as intriguing as they are important. Here just a few by way of illustration:
Time travel, superluminal travel, wormholes, teleportation
• Extraterrestrial intelligence and alien civilizations
Artificial intelligence, planetary brains, the universe as a computer, simulated worlds
• Non-anthropocentric viewpoints
Synthetic biology, genetic engineering, developing nanotechnologies
Eco/infrastructure/meteorite-impact disaster scenarios
• Future scenarios, transhumanism, posthumanism, intelligence explosion
• Virtual worlds, cyberspace dramas
Consciousness and mind manipulation

Parts of the Scientific Article

Parts of the Scientific Article
NC State University 2004
LabWrite. Improving lab reports.

– Title

– Abstract

– Introduction [develops the rationale for your work]

– Materials and Methods

– Results

– Discussion

– Conclusion

– References

Research Report Sections

Writing a Scientific Research Paper

How to Write a Thesis Statement
A strong thesis statement justifies discussion.

Quality of Scientific Articles
Rev. Saúde Pública vol.40 n.spe São Paulo Aug. 2006

How to Write Like a Scientist
March 23, 2012

How to Write a Cover Letter
scientific idioms

Pale Blue Dot: A Vision of the Human Future in Space

7 Billion People And Trillions Of Creatures To Be Photographed Together On July 19
by Robert Krulwich
June 27, 2013

Carl Sagan thought, how small we look, how small we are — which inspired him to write his eloquent Pale Blue Dot meditation, which, if you haven’t read it lately, take a minute and a half to look at this short version … Carl Sagan himself is narrating.

Pale Blue Dot: A Vision of the Human Future in Space
by Carl Sagan
Ballantine Books; Reprint edition (September 8, 1997)

Juno probe captures movie of Earth-Moon ‘dance’
10 December 2013

Earth from the International Space Station

Earth from the International Space Station
June 12, 2103

A view of Earth from the Cupola on the earth-facing side of the International Space Station is seen in this NASA handout photo taken June 12, 2103.
Visible in the top left foreground is a Russian Soyuz crew capsule.
In the lower right corner, a solar array panel can be seen.