Deterministic direct reprogramming of somatic cells to pluripotency

Eliminating Mbd3 enables the coactivators to efficiently resuscitate dormant stem-cell genes

Deterministic direct reprogramming of somatic cells to pluripotency
Nature 502, 65–70 (03 October 2013)

Somatic cells can be inefficiently and stochastically reprogrammed into induced pluripotent stem (iPS) cells by exogenous expression of Oct4 (also called Pou5f1), Sox2, Klf4 and Myc (hereafter referred to as OSKM).

The nature of the predominant rate-limiting barrier(s) preventing the majority of cells to successfully and synchronously reprogram remains to be defined.

Here we show that depleting Mbd3, a core member of the Mbd3/NuRD (nucleosome remodelling and deacetylation) repressor complex, together with OSKM transduction and reprogramming in naive pluripotency promoting conditions, result in deterministic and synchronized iPS cell reprogramming (near 100% efficiency within seven days from mouse and human cells).

Induced pluripotent stem cells
Embryonic stem cells

Stem cells: Close encounters with full potential
Kyle M. Loh & Bing Lim
Nature 502, 41–42 (03 October 2013)

Conferring stem-cell potential on mature cells is not easy. A decisive impediment to this process has now been identified, and its elimination allows almost all mature cells to efficiently adopt a stem-cell identity.

Subject terms:
Stem cells
Biological techniques
Molecular biology