Junqueira’s Basic Histology

Junqueira’s Basic Histology: Text and Atlas, Thirteenth Edition

Chapter 1. Histology & Its Methods of Study > Preparation of Tissues for Study >
“Other spatial units commonly used in histology include the nanometer (1 nm = 0.001 μm = 10−6 mm = 10−9 m) and angstrom (1 Å = 0.1 nm or 10−4 μm).”

Chapter 1. Histology & Its Methods of Study > Preparation of Tissues for Study >
freezing, unlike fixation, does not inactivate most enzymes.”

“can be used with living, cultures cells (Figure 1–5).”

“The wavelength in the electron beam is much shorter …”

“For SEM specimens are coated with metal atoms …”

“The secondary antibody labeled with peroxidase was then applied and the localized brown color ? produced histochemically with the peroxidase substrate 3,3′-diamino-azobenzidine (DAB).”

“blood vessels and other tubular structures appear in sections as round or oval shapes whose size and shape depend on …”

“transverse cuts through tubular organelles such mitochondria.”


levels of organization:
“electrostatic (salt) linkages with ionizable radicals of molecules in tissues.”
“an antibody made against the tissue protein of interest” {in ECM}
“If the cell or tissue antigen of interest is detected by …” {in ECM}


represented in plural form:
“clearing solvents such as toluene dissolve cell lipids in fixed tissues”
“Many GAGs are synthesized while attached to a core protein and are part of a class of macromolecules called proteoglycans”
content of anionic carboxyl and sulfate groups
“electrostatic interactions underlying acidophilia”
“frontiers of light microscopy ”
“the greater density of microfilaments at the cell periphery”
“structures made of highly organized subunits.” -> structure made of highly organized subunits
“substances containing highly oriented molecules” – “substance containing highly oriented molecules
“the interaction of tissue components with beams of electrons.”
“cluster of cells
“Hybridization at stringent conditions …”

represented in its usual form (but an issue when using dictionaries):
“stain more readily with basic dyes and are termed basophilic”

tokenization issue:
“periodic acid-Schiff (PAS) reagent”

multi-word concept:
“transformation of 1,2-glycol groups present in the sugars into aldehyde residues”

“Basophilic or PAS-positive material can be …”
“as well as fluorescence, phase-contrast, differential interference, confocal, and polarizing microscopy”
” magnifying power of the objective and ocular lenses” -> “magnifying power of the ocular lens”
” the power of the bright-field and other light microscopes”
“when passing through cellular and extracellular structures”


Lipid-rich structures [thing] of cells are best revealed with …”
“Cryofracture has been particularly useful in the study of membrane structure [arrangement].”
“an idea of the whole composition and structure [arrangement] of a cell or tissue”
“The TEM allows the observation of cells with all its internal structures [thing]
“… because many tissue structures [thing] are thicker than the section. Round structures [thing] seen microscopically may be portions of spheres or tubes. ”
“Because structures [thing] in a tissue have different orientations, …”


adj, v have authority==”j”

“microscope‘s resolving power”

“when a structure‘s three-dimensional volume is cut into …”
“… are used to preserve tissue structure by …”



cross-linking and denaturing proteins” -> “peptide cross-linking [GO]”



“the viewer must always keep in mind that components …” [OAAD]


adjectives that end in “-ed” (pattern: A N):
aminated sugar
cleared tissue
fixed tissue
impregnated tissue
ionized amino group
specialized method …
stained preparations
striated muscle

adjectives that end in “-ing” (pattern: A N):
magnifying power
polarizing microscopy

“the quality of its objective lens” -> “objective lens quality” (G+)

“… allows them to be digitally reconstructed into a 3D image.” -> digital reconstruction

UV is used as a noun:
“… emitting a characteristic blue fluorescence under UV.

“The ability to rotate the direction of vibration of polarized light is called birefringence and is a feature of crystalline substances ”

challenge for KE:
“repetitive, periodic macromolecular structure”

“collected on small metal grids”

longest concepts:
“axis of the light emerging from the polarizer” {8}
“artificial spaces between cells and other tissue component” -> “artificial space between a cell and other tissue component” {9}
“impossibility of differentially staining all tissue components on one slide” -> N/A
“a series of processes that began with collecting the tissue and ended with mounting a coverslip on the slide” {19}

“digoxigenin-labeled complementary DNA (cDNA)”

see also:

Introduction to Confocal Microscopy

Freeze fracture and etching

Explant culture